About Sequence Analysis

About Sequence Analysis

About Sequence Analysis

We accept only NGS sequencing of the libraries you have prepared.

Sample acceptance conditions

Sample acceptance conditions

Flow Cell Type

TypeA :

Novaseq 6000 S4 flowcell v1.5 (200 cycles, 67bp + 155bp paired-end)

¥38,500 / 100 million clusters (200 million paired-end leads)

TypeB :

Novaseq 6000 S4 flowcell v1.5 (300 cycles, 151bp + 155bp paired-end)

¥44,000 / 100 million clusters (200 million paired-end leads)

Required sample volume
(per billion reads)

Liquid volume : 30 ul or more

Concentration: 10 nM (quantified by qPCR) / 10 mM Tris HCl, pH8.5

*Suspend in 10 mM Tris-HCl, pH 8.5

* In DNA low binding tube screw cap to prevent library loss (sartorius recommended)

Provide information about the library you are sending us

・ 

Library quantitative data by qPCR

・ 

Library size distribution and concentration information in bioanalyzers, Tapestation, MultiNA, etc.

・ 

Unique-dual index information

* If a sample does not meet our recommended criteria, we may stop the analysis or ask you to resubmit the sample.

* Quantification by qPCR can be performed at our company (at an additional cost).

*サンプルQCとして、iSeqによるプレランサービスを実施致します

Number of samples

・ 

Acceptable from 1 sample

・ 

Multiple pooled samples are also acceptable

* poolしたライブラリ全体のシーケンスリード量は-10%を保証致します

* Variations in output within a pooled library are not guaranteed.

Lead Time

Please contact us