Sequence Analysis

Sequence Analysis

Sequence Analysis

About Sequence Analysis

About Sequence Analysis

About Sequence Analysis

We accept only NGS sequencing of the libraries you have prepared.

Sample acceptance conditions

Sample acceptance conditions

Flow Cell Type

TypeA :

Novaseq 6000 S4 flowcell v1.5 (200 cycles, 67bp + 155bp paired-end)

¥38,500 / 100 million clusters (200 million paired-end leads)

TypeB :

Novaseq 6000 S4 flowcell v1.5 (300 cycles, 151bp + 155bp paired-end)

¥44,000 / 100 million clusters (200 million paired-end leads)

Required sample volume
(per billion reads)

Liquid volume : 30 ul or more

Concentration: 10 nM (quantified by qPCR) / 10 mM Tris HCl, pH8.5

*Suspend in 10 mM Tris-HCl, pH 8.5

* In DNA low binding tube screw cap to prevent library loss (sartorius recommended)

Provide information about the library you are sending us

・ 

Library quantitative data by qPCR

・ 

Library size distribution and concentration information in bioanalyzers, Tapestation, MultiNA, etc.

・ 

Unique-dual index information

* If a sample does not meet our recommended criteria, we may stop the analysis or ask you to resubmit the sample.

* Quantification by qPCR can be performed at our company (at an additional cost).

*サンプルQCとして、iSeqによるプレランサービスを実施致します

Number of samples

・ 

Acceptable from 1 sample

・ 

Multiple pooled samples are also acceptable

* poolしたライブラリ全体のシーケンスリード量は-10%を保証致します

* Variations in output within a pooled library are not guaranteed.

Lead Time

Please contact us