Sequence Analysis
We accept only NGS sequencing of the libraries you have prepared.
Flow Cell Type
TypeA :
Novaseq 6000 S4 flowcell v1.5 (200 cycles, 67bp + 155bp paired-end)
¥38,500 / 100 million clusters (200 million paired-end leads)
TypeB :
Novaseq 6000 S4 flowcell v1.5 (300 cycles, 151bp + 155bp paired-end)
¥44,000 / 100 million clusters (200 million paired-end leads)
Required sample volume
(per billion reads)
Liquid volume : 30 ul or more
Concentration: 10 nM (quantified by qPCR) / 10 mM Tris HCl, pH8.5
*Suspend in 10 mM Tris-HCl, pH 8.5
* In DNA low binding tube screw cap to prevent library loss (sartorius recommended)
Provide information about the library you are sending us
・
Library quantitative data by qPCR
・
Library size distribution and concentration information in bioanalyzers, Tapestation, MultiNA, etc.
・
Unique-dual index information
* If a sample does not meet our recommended criteria, we may stop the analysis or ask you to resubmit the sample.
* Quantification by qPCR can be performed at our company (at an additional cost).
*サンプルQCとして、iSeqによるプレランサービスを実施致します
Number of samples
・
Acceptable from 1 sample
・
Multiple pooled samples are also acceptable
* poolしたライブラリ全体のシーケンスリード量は-10%を保証致します
* Variations in output within a pooled library are not guaranteed.
Lead Time
Please contact us