Trace Sample RNA-seq
By analyzing mRNA sequences in cells, this technique allows quantification of expression levels in all transcripts in the cell.
・
Highly sensitive cDNA amplification system enables analysis of even minute amounts of samples. (1,000 cells, 10 ng or more)
* If less than 10 ng, please consult us.
・
By lysing cell samples with our specified lysis solution, the RNA preparation process can be omitted.
・
5' end sequencing can be performed to reduce sequencing costs.
The mRNA in the sample is concentrated by magnetic beads with oligo-dT primer attached, and cDNA synthesized by reverse transcription reaction is amplified and sequenced by NGS (5'-end Tag sequencing).
How to request
Sample acceptance
・
Purified total RNA
・
Cell lysate dissolved in our designated lysis solution
・
Cryopreserved tissue specimens treated with RNA stabilization
Send samples to
〒277-0882
千葉県柏市柏の葉6丁目6番2号
三井リンクラボ柏の葉1 304号室
TEL:04-7192-8732
ImmunoGeneTeqs, Inc.
How to send samples
・
Please make sure that the sample is dry and leak-free, and send it to the shipping address below. (Available until 5:00 p.m. on weekdays)
・
If the sample to be sent is RNA, please send it frozen with enough dry ice to keep it frozen.
* Please send by next day except for remote areas.
Lead Time
From sample receipt
2.5 months
Deliverables
・
Work report
・
Complete sequence data
・
Mapping result files, etc.
We can create a gene expression table for each cell from sequence data.
Reference price
Number of samples
Price (incl. tax)
1
¥77,000
4
¥286,000
8
¥497,200
Additional analysis
Clustering using WGCNA and gene ontology analysis are also available as options.