By analyzing mRNA sequences in cells, this technique allows quantification of expression levels in all transcripts in the cell.
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Highly sensitive cDNA amplification system enables analysis of even minute amounts of samples. (1,000 cells, 10 ng or more)
* If less than 10 ng, please consult us.
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By lysing cell samples with our specified lysis solution, the RNA preparation process can be omitted.
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5' end sequencing can be performed to reduce sequencing costs.
The mRNA in the sample is concentrated by magnetic beads with oligo-dT primer attached, and cDNA synthesized by reverse transcription reaction is amplified and sequenced by NGS (5'-end Tag sequencing).
How to request
Sample acceptance
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Purified total RNA
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Cell lysate dissolved in our designated lysis solution
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Cryopreserved tissue specimens treated with RNA stabilization
Send samples to
1F Building 17, Tokyo University of Science, Noda Campus
2669 Yamazaki, Noda, Chiba 278-0022, Japan
ImmunoGeneTeqs, Inc.
How to send samples
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Please make sure that the sample is dry and leak-free, and send it to the shipping address below. (Available until 5:00 p.m. on weekdays)
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If the sample to be sent is RNA, please send it frozen with enough dry ice to keep it frozen.
* Please send by next day except for remote areas.
Lead Time
From sample receipt
2.5 months
Deliverables
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Work report
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Complete sequence data
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Mapping result files, etc.
We can create a gene expression table for each cell from sequence data.
Reference price
Number of samples
Price (incl. tax)
1
¥77,000
4
¥286,000
8
¥497,200
Additional analysis
Clustering using WGCNA and gene ontology analysis are also available as options.
If you would like to request an analysis, please fill out and submit the hearing form at
We will prepare a quotation based on the information you send us.