What is RNA-seq?

What is RNA-seq?

By analyzing mRNA sequences in cells, this technique allows quantification of expression levels in all transcripts in the cell.

ImmunoGeneTeqs

Features of RNA-seq

ImmunoGeneTeqs

Features of RNA-seq

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Highly sensitive cDNA amplification system enables analysis of even minute amounts of samples. (1,000 cells, 10 ng or more)

 

* If less than 10 ng, please consult us.

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By lysing cell samples with our specified lysis solution, the RNA preparation process can be omitted.

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5' end sequencing can be performed to reduce sequencing costs.

Analysis Method

Analysis Method

The mRNA in the sample is concentrated by magnetic beads with oligo-dT primer attached, and cDNA synthesized by reverse transcription reaction is amplified and sequenced by NGS (5'-end Tag sequencing).

Sample acceptance

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Purified total RNA

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Cell lysate dissolved in our designated lysis solution

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Cryopreserved tissue specimens treated with RNA stabilization

Send samples to

1F Building 17, Tokyo University of Science, Noda Campus
2669 Yamazaki, Noda, Chiba 278-0022, Japan

ImmunoGeneTeqs, Inc.

How to send samples

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Please make sure that the sample is dry and leak-free, and send it to the shipping address below. (Available until 5:00 p.m. on weekdays)

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If the sample to be sent is RNA, please send it frozen with enough dry ice to keep it frozen.

* Please send by next day except for remote areas.

Lead Time

From sample receipt

2.5 months

Deliverables

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Work report

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Complete sequence data

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Mapping result files, etc.

We can create a gene expression table for each cell from sequence data.

Reference price

Number of samples

Price (incl. tax)

1

¥77,000

4

¥286,000

8

¥497,200

Additional analysis

Clustering using WGCNA and gene ontology analysis are also available as options.

RNA-seq
Analysis Overview Hearing Form

RNA-seq
Analysis Overview Hearing Form

If you would like to request an analysis, please fill out and submit the hearing form at

We will prepare a quotation based on the information you send us.